Heterogeneous elastic response of human lung microvascular endothelial cells to barrier modulating stimuli.

TitleHeterogeneous elastic response of human lung microvascular endothelial cells to barrier modulating stimuli.
Publication TypeJournal Article
Year of Publication2013
AuthorsArce FTerán, Meckes B, Camp SM, Garcia JGN, Dudek SM, Lal R
JournalNanomedicine
Volume9
Issue7
Pagination875-84
Date Published2013 Oct
ISSN Number1549-9642
KeywordsActins, Cell Line, Cytoskeleton, Elasticity, Endothelial Cells, Finite Element Analysis, Humans, Lung, Lysophospholipids, Microvessels, Models, Biological, Sphingosine, Stress, Mechanical, Thrombin
Abstract

<p><b>UNLABELLED: </b>In this study we employ atomic force microscopy, supported by finite element analysis and fluorescence microscopy, to characterize the elastic properties accompanying cytoskeletal structural rearrangements of lung microvascular endothelial cells in response to barrier altering stimuli. Statistical analysis of elasticity data obtained from multiple cells demonstrates a heterogeneous cellular elastic response to barrier-enhancing and barrier-disrupting agents; sphingosine 1-phosphate (S1P) and thrombin, respectively. A small but detectable (10%) increase in the average elastic modulus of all cells is observed for S1P, which is accompanied by a corresponding significant decrease in cell thickness. Variable effects of thrombin on these parameters were observed. To account for possible substrate effects in our elasticity analysis, we analyzed only the low-force sections of the force-displacement curves and utilized a finite-thickness correction to the Hertzian model. Our finite element analysis results substantiate this approach. The heterogeneous elastic behavior correlates with differential cytoskeletal rearrangements observed with fluorescence microscopy.</p><p><b>FROM THE CLINICAL EDITOR: </b>This team of investigators employed atomic force microscopy coupled with finite element analysis and fluorescence microscopy to characterize the elastic properties accompanying cytoskeletal structural rearrangements of lung microvascular endothelial cells in response to barrier altering stimuli, demonstrating the validity of their approach.</p>

DOI10.1016/j.nano.2013.03.006
Alternate JournalNanomedicine
PubMed ID23523769
PubMed Central IDPMC3762941
Grant ListR01 HL 88144 / HL / NHLBI NIH HHS / United States
5R01DA025296-05 / DA / NIDA NIH HHS / United States
R01 DA025296 / DA / NIDA NIH HHS / United States
P01 HL 58064 / HL / NHLBI NIH HHS / United States
5R01DA024871-15 / DA / NIDA NIH HHS / United States
R01 HL088144 / HL / NHLBI NIH HHS / United States
R01 DA024871 / DA / NIDA NIH HHS / United States
P01 HL058064 / HL / NHLBI NIH HHS / United States