Role of calcineurin in thrombin-mediated endothelial cell contraction.

TitleRole of calcineurin in thrombin-mediated endothelial cell contraction.
Publication TypeJournal Article
Year of Publication2009
AuthorsKolozsvári B, Szíjgyártó Z, Bai P, Gergely P, Verin A, Garcia JGN, Bakó E
JournalCytometry A
Volume75
Issue5
Pagination405-11
Date Published2009 May
ISSN Number1552-4930
KeywordsAnimals, Calcineurin, Calcineurin Inhibitors, Catalytic Domain, Cattle, Cell Line, Cell Survival, Cyclosporine, Cytoskeleton, Endothelial Cells, Immunosuppressive Agents, Stress Fibers, Tacrolimus, Thrombin, Transfection
Abstract

<p>Barrier function and shape changes of endothelial cells (EC) are regulated by phosphorylation/dephosphorylation of key signaling and contractile elements. EC contraction results in intercellular gap formation and loss of the selective vascular barrier to circulating macromolecules. EC dysfunction elicited by thrombin was found to correlate with actin microfilament redistribution. It is known that calcineurin (Cn) is involved in thrombin-induced EC dysfunction because inhibition of Cn potentiates PKC activity and the phosphorylation state of EC myosin light chain is also affected by Cn activity. Immunofluorescent detection of Cn catalytic subunit (CnA) isoforms coexpressed with GFP was visualized on paraformaldehyde (PFA) fixed bovine pulmonary artery endothelial cells (BPAEC). Actin microfilaments were stained with Texas Red-phalloidin. Cytotoxic effects of transfections or treatments and the efficiency of transfections were assessed by flow cytometry. Treatment of BPAEC with Cn inhibitors (cyclosporin A and FK506) hindered recovery of the cells from thrombin-induced EC dysfunction. Inhibition of Cn in the absence of thrombin had no effect on cytoskeletal actin filaments. We detected attenuated thrombin-induced stress fiber formation and changes in cell shape only when cells were transfected with constitutively active CnA and not with various CnA isoforms. Flow cytometry (FCM) analysis has proved that cytotoxic effect of treatments is negligible. We observed that Cn is involved in the recovery from thrombin-induced EC dysfunction. Inhibition of Cn caused prolonged contractile effect, while overexpression of constitutively active CnA resulted in reduced thrombin-induced stress fiber formation.</p>

DOI10.1002/cyto.a.20707
Alternate JournalCytometry A
PubMed ID19235203
PubMed Central IDPMC3884896
Grant ListR01 HL067307 / HL / NHLBI NIH HHS / United States
R01 HL067307-08 / HL / NHLBI NIH HHS / United States